This class covers the preparation of tissues for histological examination. Topics covered include recording specimen data, tissue collection, fixation, decalcification, dehydration, infiltration, embedding, sectioning, and microscopic examination. Secondary topics include staining, interpretation of sections, and quality control procedures.
Prerequisite Courses
Prerequisites
Credit for BIOL 100 or BIOL 172/172L, BOT 101 or above, or PHYL 141 or consent of instructor.
Course Outcomes
- Properly dissect and preserve specimens for histological analysis and record pertinent specimen info.
- Dehydrate, infiltrate, and embed specimens.
- Produce sections of suitable thickness and quality for diagnostic use.
- Properly stain and coverslip specimens.
- Identify tissue types and histological artifacts in order to improve quality of sections.